PKM2 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: PKM2 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-GV5S
• Supplier Catalog Number: 600-401-GV5S
• Alternative Catalog Number: ROC-600-401-GV5S
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human
• Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, OIP3, OIP-3, PK2, PK3

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 5315
• UniProt: P14618
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:50,000, WB: 1.0 ug/ml
• Application Notes: This affinity purified antibody has been tested for use in western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or e

Images

Western Blot of Rabbit Anti-PKM2 antibody. Lane 1 and 2: iPSC lysate. Lane 3 and 4: myeloid lysate (iPSC derived). Load: 100 µg per lane. Primary antibody: PKM2 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Odyssey blocking buffer for 120 min at RT. Predicted/Observed size: 57 kda for PKM2. Other band(s): PKM2 splice variants and isoforms as PKM2 is a highly modified protein.