Affinity purified Anti-Ffar4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the N-terminus portion of mouse Ffar4 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:50,000, WB: 1.0 ug/ml
Application Notes:
Anti-Ffar4 Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~40-48 kDa in size corresponding to Ffar4 by western blotting in the appropriate cell lysate or e
Western Blot of Rabbit Anti-Ffar4 antibody. Lane 1: Ladder Opal Prestained (p/n MB-210-0500). Lane 2: Raw 264.7 WCL (p/n W10-001-369). Lane 3: NIH/3T3 WCL (p/n W10-000-358). Lane 4: PC-12 WCL (p/n W12-001-GL9). Lane 5: Ladder Opal Prestained (p/n MB-210-0500). Load: 35 µg per lane. Primary antibody: Ffar4 antibody at 1:1000 for overnight at 4C. Secondary antibody: Gt-a-Rb HRP secondary antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: (p/n MB-073) BlockOut Universal Blocking Buffer for 30 min at RT. Predicted/Observed size: 40.8 kDa, observed ~48 kDa band due to glycosylation of Ffar4 protein.
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