ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-term of human ACSS2.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-ACSS2, rabbit anti ACSS2, Acetyl-coenzyme A synthetase (cytoplasmic), Acetate-CoA ligase, Acetyl-CoA synthetase, ACS, AceCS, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme, ACSS2, ACAS2
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, WB: 1.0 ug/ml
Application Notes:
Anti-ACSS2 Antibody has been tested in Western Blot and ELISA. Expect a band at approximately 76 kDa in western blot using appropriate lysates. Positive control whole cell lysates used Hep-G2 at 1µg/mL for WB. Although not tested, this antibody is likely
Western Blot of Rabbit anti-ACSS2 antibody. Lane 1: MW ladder Opal pre-stained (p/n MB-210-0500). Lane 2: HEP-G2 WCL (p/n W09-001-GJ5). Load: 10 ug per lane. Primary antibody: ACSS2 antibody at 1:1000 for overnight at 4C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 30 min at RT. Block: BlockOut (p/n MB-073) for 30 min at RT. Predicted/Observed size: Expect 76 kDa for ACSS2 protein.
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