TAF10 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal portion of human TAF10.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: User Optimized, WB: 1.0 ug/ml
Application Notes:
Anti-TAF10 Antibody has been tested for use in ELISA, Dot Blot, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to TAF10 protein by western blotting in t
Western Blot of Rabbit Anti-TAF10 antibody. Lane 1: Opal Pre-stained Ladder (p/n MB-210-0500). Lane 2: Hela WCL (p/n W09-000-364). Lane 3: HeLa nuclear extract (p/n W09-001-367). Lane 4: HEK293 WCL (p/n W09-000-365). Lane 5: PANC-1 WCL (p/n W09-001-GM2). Lane 6: Mouse brain WCL (p/n W10-001-T004). Lane 7: Opal Pre-stained Ladder (p/n MB-210-0500). Load: 35 µg per lane. Primary antibody: TAF10 antibody at 1:1000 for overnight at 4C. Secondary antibody: rabbit secondary HRP antibody (p/n 611-103-122) at 1:70,000 for 45 min at RT. Block: (p/n MB-070) overnight at 4C. Predicted/Observed size: 21 kda for TAF10.
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