Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Mouse
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5000, IP: 1 µg/mL, WB: 1 µg/mL
Application Notes:
Affinity purified Bach-2 antibody has been tested for use in ELISA, Immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91-140 kDa in size corresponding to Bach2 prote
Western Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 µg per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted (native)/Observed (over-expressed) size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none.
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