Anti-POL II antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminal portion of human POL II conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugation:
Unconjugated
Alternative Names:
RPO2, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, RPB1, POLR2A,
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:50,000, ChIP: User Optimized, IF Microscopy: User Optimized, WB: 1:1000
Application Notes:
Anti-POL II antibody has been tested in ChIP and ELISA, and is likely suitable for western blotting and immunofluorescence assays. Positive controls used in ChIP were EGF stimulated NR4A1 5, NR4A1 3 and FOS 3. The negative control was total rabbit IgG.
Chromatin Immunoprecipitation of Rabbit Anti-Pol II Antibody. Chromatin from three million formaldehyde cross-linked HeLa cells (total volume 1.0mL) was used with 2.5µg of Anti-Pol II was used to IP DNA to test in three different conditions, NR4A1 5, NR4A1 3 and FOS 3 starved and EGF stimulated with total rabbit IgG used as control.
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