HDAC4 (internal) Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: HDAC4 (internal) Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-J20
• Supplier Catalog Number: 600-401-J20
• Alternative Catalog Number: ROC-600-401-J20
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Mouse, Primate, Rat
• Immunogen: HDAC4 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region surrounding 600-650aa of human HDAC4.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-HDAC-4 antibody, HDAC4, HDAC 4, HD 4 antibody, histone deacetylase 4 antibody, Histone deacetylase-4, HD4 antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 9759
• UniProt: P56524
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:60,000, IHC: 1 mg/mL, IF Microscopy: 1:100-1:500, WB: 1 mg/mL
• Application Notes: Anti-HDAC4 antibody has been tested in Western Blot and is useful for ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~140 kDa corresponding to the appropriate cell lysat

Images

Western Blot of Rabbit anti-HDAC4 antibody. Lane 1: Mouse brain homogenate. Lane 2: Mouse brain homogenate blocked with peptide. Load: 30 µg per lane. Primary antibody: HDAC4 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 140 kDa, 98 kDa, and 55 kDa for HDAC4. Other band(s): HDAC4 splice variants and isoforms.