HDAC7 (N-terminus) Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: HDAC7 (N-terminus) Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-J22
• Supplier Catalog Number: 600-401-J22
• Alternative Catalog Number: ROC-600-401-J22
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse, Primate, Rat
• Immunogen: HDAC7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-HDAC-7 antibody, HDAC7, HDAC 7, HD 7 antibody, histone deacetylase 7 antibody, Histone deacetylase-7, HD7A antibody, histone deacetylase 7A antibody, Histone deacetylase-7A, HDAC7A, HDAC 7A, HDAC-7A, HD-7A

Product Properties

• Clonality: Polyclonal
• Concentration: 1.16 mg/ml by UV absorbance at 280 nm
• NCBI: 51564
• UniProt: Q8WUI4
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF Microscopy: 1:100-1:500, WB: 1 µg/ml
• Application Notes: Anti-HDAC7 antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or ext

Images

Western Blot of Rabbit anti-HDAC7 antibody. Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 µg per lane. Primary antibody: HDAC7 antibody at 1µg/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~103kDa/ ~103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms ~70 and 40kDa.