Kmo Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Kmo Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-J26
• Supplier Catalog Number: 600-401-J26
• Alternative Catalog Number: ROC-600-401-J26
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Mouse
• Immunogen: KMO affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding 200-300aa of the mouse KMO chain.
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-KMO antibody, kynurenine 3-monooxygenase, kynurenine 3-hydroxylase

Product Properties

• Clonality: Polyclonal
• Concentration: 1.07 mg/ml by UV absorbance at 280 nm
• NCBI: 8564
• UniProt: O15229
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Mouse
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF Microscopy: 1:100-1:500, WB: 1 µg/ml
• Application Notes: Anti-KMO antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~58 kDa corresponding to the appropriate cell lysate or extract.

Images

Western Blot of Rabbit anti-KMO antibody. Lane 1: Brain Extract. Load: 10 µg per lane. Primary antibody: KMO antibody at 1µg/mL for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa for KMO. Other band(s): Other bands at lower molecular weights. These bands are all specifically blocked by KMO peptide.