Anti-Beta Amyloid 42 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of AB42.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1: 10,000, WB: 1ug/ml
Application Notes:
Anti-Beta Amyloid 1-42 antibody has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~86kDa corresponding to the appropriate cell lysate or extract.
ELISA results of purified Polyclonal anti-beta amyloid 42 antibody tested against BSA-conjugated beta amyloid 42 peptide (green) and BSA-conjugated beta amyloid 40 peptide (blue). Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060), Goat anti-Rabbit IgG HRP conjugated (611-103-122), and TMB substrate (p/n TMBM-1000).
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