Anti-Caveolin 1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide at the N-terminus of human Caveolin-1 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1: 10,000, WB: 1ug/ml
Application Notes:
Anti-Caveolin1 antibody has been tested in western blot and is useful for ELISA and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~21kDa corresponding to the appropriate cell lys
Western Blot of Rabbit Anti-Caveolin 1 Antibody. Lane 1: PG13 Whole Cell Lysate - Reduced [5µg]. Lane 2: PG13 Whole Cell Lysate - Reduced [1µg]. Lane 3: Opal Prestained MW Marker Lane 4: PG13 Cytosolic Extract - Reduced [1µg]. Lane 5: PG13 Cytosolic Extract - Reduced [5µg]. Primary Antibody: Anti-Caveolin-1 at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG Peroxidase Conjugated (p/n 611-103-122) at 1:70,000 for 1 hour at RT. Block: BlockOut Blocking Buffer (p/n MB-073) overnight at 2-8C. Predicted MW: ~21kDa. Exposure: 5 sec.
* VAT and and shipping costs not included. Errors and price changes excepted
