SMAD2 pS465 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.
Conjugation:
Unconjugated
Alternative Names:
rabbit anti-SMAD2 pS465 pS467 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000, WB: 1:1,000 - 1:3,000
Application Notes:
Anti-SMAD2 pS465 pS467 affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Smad2 protein by
Western Blot of Rabbit anti-Smad2 pS465pS467 antibody. Lane 1: MDA-MB-231 cells. Lane 2: MDA-MB-231 cells treated with TGF-ß1 for 1h. Load: 20 µg per lane. Primary antibody: Smad2pS465pS467 antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 52.3 kDa for Smad2pS465pS467. Other band(s): ~85kDa non-specific band.
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