SARS-CoV-2 Nucleocapsid (N) Protein Antibody, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-600-401-MS4
Supplier Catalog Number:
600-401-MS4
Alternative Catalog Number:
ROC-600-401-MS4
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
ELISA
Species Reactivity:
Virus
Immunogen:
Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
Conjugation:
Unconjugated
Alternative Names:
2019-nCoV, COVID-19, N-protein antibody, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2, rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, sars-cov-2
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
SARS
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:500,000-1:600,000, WB: User Optimized
Application Notes:
Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and is suitable in Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-C
Surface Plasmon Resonance (SPR) Data of Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Antibody. SARS-CoV-2 Nucleocapsid protein was immobilized on a carboxymethyldextran sensor chip. Ten (10) dilutions of Anti-SARS-CoV-2 Nucleocapsid (N) Antibody (colors) were flowed across the chip at 30 µL/min with 240 s contact time and then 720 s dissociation time. Fitted line (black) constants are: kon = 2.2 x 104 M-1 s-1 and koff = 3.3 x 10-4 s-1. The Equilibrium Dissociation constant (KD) of the antibody on several assays was 15 nM. (Average primary antibody KD = 1 µM).
Binding mode and kinetic analysis of Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody (p/n 600-401-MS4). Using Octet R4, SARS COV-2N was immobilized at 20 µg/mL on an AR2G biosensor and exposed to Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody over a range of 0.7 nM - 5.6 µM. Each curve represents the acquired signal of its protein concentration. The interaction fits to a bivalent binding site kinetic model. The association constant (ka=1.7 x 10 4 M -1 s -1 and dissociation constant (kd=7.9 x 10 -5 s -1) values are depicted in the graph. The assay was performed as per the manufacturers protocol.
ELISA results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5 µg of SARS CoV-1 (N) Protein [Red Line], SARS CoV-2 (N) Protein [Green Line], MERS N [Blue Line]. The starting dilution of antibody was 0.5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1 N IC50: 3ng/mL, SARS CoV-2 N IC50: 2ng/mL, MERS N IC50: 25mg/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).
Fitting of Kinetic Data to bivalent binding site model. Steady state signals are fitted to a bivalent binding site model. A 4-parameter non-linear regression curve was used for the integration of the data. The Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody (p/n 600-401-MS4) presents a KD= 4.7 µM (affinity constant) to SARS-CoV-2 Nucleocapsid (N) Protein.
ELISA results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein Variants. Each well was coated in duplicate with 0.5 µg/mL of SARS CoV-2 Canonical Nc [Red Line], SARS CoV-2 Omicron Nc [Green Line], SARS CoV-2 Omicron BA.2 Nc [Blue Line]. The starting dilution of antibody was 0.55 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-2 Nc IC50: 4 ng/mL, SARS CoV-2 Omicron Nc IC50: 5 ng/mL, SARS CoV-2 BA.2 Nc IC50: 5 ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).
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