SARS-CoV-2 Spike Protein (RBD) Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: SARS-CoV-2 Spike Protein (RBD) Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-MS8
• Supplier Catalog Number: 600-401-MS8
• Alternative Catalog Number: ROC-600-401-MS8
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF
• Species Reactivity: Virus
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the SARS Coronavirus Spike Protein within the Receptor Binding Domain (RBD).
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV, COVID-19, S glycoprotein, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2), Spike glycoprotein antibody, rabbit anti-SARS CoV 2 Spike Protein Receptor Binding Domain Antibody, sars-cov-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1.13 mg/mL by UV absorbance at 280 nm
• NCBI: 908947
• UniProt: P0DTC2
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: SARS
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:150,000 - 1:450,000, IF Microscopy: 1:1000
• Application Notes: Anti-SARS-CoV-2 Spike protein purified antibody has been tested for use in ELISA and IF. This antibody shows reactivity to recombinant SARS COV-2, S1 subunit protein and recombinant SARS COV-2 whole spike protein. Specific conditions for reactivity shoul

Images

ELISA Results of Rabbit Anti-SARS Cov-2 Spike (RBD) Antibody. Each well was coated in duplicate with 0.5µg of rec SARS-Cov-2 S1 subunit [RED line], rec SARS Cov-2 whole spike protein [GREEN line], rec SARS Cov-2 spike glyo-S2 subunit protein [BLUE line], and rec MERS Cov spike/S1 protein [PURPLE line]. The working dilution for SARS-Cov-2 S1 is 1:419,000 and SARS Cov-2 whole spike is 1:12,700. The starting dilution of antibody was 10µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS-Cov-2 S1 subunit IC50: 2ng/mL and SARS Cov-2 whole spike protein IC50: 79ng/mL.

Immunofluorescence assay using Rabbit Anti-SARS-CoV-2 Spike Protein (RBD) Antibody, showing viral protein detection. Vero E6 cells were either infected with the SARS-CoV-2 Washington isolate (INF) at an MOI of 0.1 or uninfected (UNI) for 24 hours. The cells were then fixed in 4% PFA and stained overnight at 4C with primary antibodies directed against SARS-CoV-2 Spike (S) Protein at 1:1000 dilution. Imaged using an anti-rabbit secondary conjugated to AlexaFluor 568 [red] and Nuclear Counterstain DAPI [blue]. Image Courtesy of Mohsan Saeed Lab/Da-Yuan Chen, National Emerging Infectious Diseases Laboratories (NEIDL), Boston University.

ELISA Results of Rabbit Anti-SARS Cov-2 Spike (RBD) Antibody. Each well was coated in duplicate with 0.5µg of BSA conjugated SARS-Cov-2 S1 (N-Term) [RED line] and BSA conjugated SARS-Cov-2 S1 (C-Term) [GREEN line]. The working dilution for BSA conjugated SARS-Cov-2 S1 (N-Term) is 1:239,000 and BSA conjugated SARS-Cov-2 S1 (C-Term) is 1:98,800. The starting dilution of antibody was 0.55µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS-Cov-2 S1 (N-Term) IC50: 4ng/mL and SARS-Cov-2 S1 (C-Term) IC50: 10ng/mL.