Apelin Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: Apelin Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-R09
• Supplier Catalog Number: 600-401-R09
• Alternative Catalog Number: ROC-600-401-R09
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Apelin affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human Apelin.
• Conjugation: Unconjugated
• Alternative Names: APJ endogenous ligand,

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by UV absorbance at 280 nm
• NCBI: 8862
• UniProt: Q9ULZ1
• Buffer: 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: WB: 0.5 µg/mL
• Application Notes: Anti-Apelin is tested for Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~8.6 kDa corresponding to the appropriate cell lysate or extract.

Images

Western Blot of Rabbit Anti-Apelin antibody. Lane 1: U87 Cell Lysate. Lane 2: MCF-7 Cell Lysate. Lane 3: HELA Cell Lysate. Lane 4: MM453 Cell Lysate. Load: 35 µg per lane. Primary antibody: Apelin antibody at 1 ug/ml for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 1.5 hr at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~9 kDa for Apelin. Other band(s): none.