Anti-Phospho-Ser421 MeCP2 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser421 of mouse MeCP2 protein conjugated to KLH.
Anti-Phospho-Ser421 MeCP2 Antibody is tested for Western Blots and is specific for the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421. Immunolabeling of the MeCP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not
Western blot of Anti-MeCP2 pS421 Antibody. Mouse whole brain lysate showing specific immunolabeling of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
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