Anti-Aak1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 18 amino acid synthetic peptide near the C-terminus of the human Aak1.
Conjugation:
Unconjugated
Alternative Names:
Aak1 Antibody, KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:10,000 - 1:20,000, WB: 1-2 µg/mL
Application Notes:
Anti-Aak1 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~104 kDa in size corresponding to Aak1 protein by Western Blotting in the appropriate cell
Western Blot of Aak1 antibody. Lane A: A20 cell lysate at 1 µg/mL. Lane B: A20 cell lysate at 2 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103.8 kDa, ~86 kDa for Aak1.
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