ATG4A Antibody, Rabbit, Polyclonal

Catalog Number: ROC-600-401-Y82
Article Name: ATG4A Antibody, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-401-Y82
Supplier Catalog Number: 600-401-Y82
Alternative Catalog Number: ROC-600-401-Y82
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Anti-ATG4A antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the C-terminus of human ATG4A.
Conjugation: Unconjugated
Alternative Names: Autophagy related 4A cysteine peptidase, APG4-A, APG4-A, AUTL2
Clonality: Polyclonal
Concentration: 1 mg/mL by UV absorbance at 280 nm
NCBI: 115201
UniProt: Q8WYN0
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form: Liquid (sterile filtered)
Target: Human
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 5 µg/mL, WB: 1 - 2 µg/mL
Application Notes: Anti-ATG4A Antibody has been tested for use in ELISA, immunohistochemistry, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 45 kDa in Western Blots of specific cell lysates and
Western Blot of Rabbit anti-ATG4A antibody. Lane A: EL4 cell lysate at 1 µg/mL. Lane B: EL4 cell lysate at 2 µg/mL. Primary antibody: ATG4A antibody overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 48 kDa, 44 kDa for ATG4A.