BAG1 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: BAG1 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-Z21
• Supplier Catalog Number: 600-401-Z21
• Alternative Catalog Number: ROC-600-401-Z21
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Anti-BAG-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from near the C-terminus of human BAG-1.
• Conjugation: Unconjugated
• Alternative Names: BAG-1 Antibody, HAP, BAG-1, RAP46, HAP, BAG family molecular chaperone regulator 1, Bcl-2-associated athanogene 1

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 573
• UniProt: Q99933
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 5 µg/mL, IF Microscopy: 20 µg/mL, WB: 1 - 2 µg/mL
• Application Notes: Anti-BAG-1 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 39 kDa in Western Blots of specif

Images

Western Blot of BAG-1 antibody in PC-3 cell lysate. Lane A: BAG-1 antibody at 1 µg/mL. Lane B: BAG-1 antibody at 2 µg/mL. Load: 35 µg per lane. Primary antibody: BAG-1 antibody at designated concentrations for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 39 kDa, 37 kDa for BAG-1. Other band(s): BAG-1 splice variants and isoforms.