BECLIN2 Antibody, Rabbit, Polyclonal

Product Overview

• Article Name: BECLIN2 Antibody, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-401-Z52
• Supplier Catalog Number: 600-401-Z52
• Alternative Catalog Number: ROC-600-401-Z52
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, IHC, WB
• Species Reactivity: Human
• Immunogen: Anti-Beclin 2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the N-terminus of human Beclin 2.
• Conjugation: Unconjugated
• Alternative Names: BECN2, BECN1L1, Beclin-1-like protein 1, Beclin 1 autophagy related pseudogene 1, BECN1P1, BECLIN 2, BECLIN-2

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 441925
• UniProt: A8MW95
• Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 20 µg/mL, IF Microscopy: 20 µg/mL, IP: 5 µg/mL, WB: 1 - 2 µg/mL
• Application Notes: Anti-Beclin 2 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 48 kDa in Western Blots of spe

Images

Western Blot of Rabbit anti-Beclin 2 antibody. Lane A: Jurkat cell lysate in the absence of blocking peptide. Lane B: Jurkat cell lysate in the presence of blocking peptide. Primary antibody: Beclin 2 antibody at 1 µg/mL overnight at 4°C. Secondary antibody: Goat anti-Rabbit HRP secondary antibody. Block: 5% BLOTTO. Predicted/Observed size: 47 kDa, 55 kDa for Beclin 2.