Collagen Type III Antibody Fluorescein Conjugated, FITC, Rabbit, Polyclonal

Catalog Number: ROC-600-402-105
Article Name: Collagen Type III Antibody Fluorescein Conjugated, FITC, Rabbit, Polyclonal
Biozol Catalog Number: ROC-600-402-105
Supplier Catalog Number: 600-402-105
Alternative Catalog Number: ROC-600-402-105
Manufacturer: Rockland Immunochemicals
Host: Rabbit
Category: Antikörper
Species Reactivity: Bovine, Human
Immunogen: Collagen Type III from human and bovine placenta
Conjugation: FITC
Alternative Names: rabbit anti-Collagen Type III antibody fluorescein conjugation, FITC conjugated rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain
Clonality: Polyclonal
Concentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 1281
UniProt: P02461
Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Form: Lyophilized
Target: Mammalian
Antibody Type: Primary Antibody
Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000
Application Notes: Anti-Collagen Type III Fluorescein Conjugated Antibody is suitable for western blotting, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Rockland anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA