0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Antibody Type:
Primary Antibody
Application Dilute:
FLISA: User Optimized, IF Microscopy: 1:500-1:2,500, WB: User Optimized
Application Notes:
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor
Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody (600-401-466). Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TBS overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.
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