Anti-MEK1 Antibody was produced in rabbits by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Target:
Human
Antibody Type:
Primary Antibody
Application Dilute:
FLISA: 1:40,000, IHC: 1:100, WB: 1µg/ml
Application Notes:
Anti-MEK 1 Fluorescein conjugated antibody has been tested by western blot and is suitable for use in ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.
Western Blot of Rabbit Anti-MEK1 (C-term) FITC Conjugated Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500) (5µL). Lane 2: MEK1 human recombinant protein (p/n 009-001-GQ3) (0.1µg). Primary Antibody: Anti-MEK1 FITC conjugated at 1µg/mL overnight at 2-8C. Secondary Antibody: none. Block: BlockOut Buffer (p/n MB-073). Expected MW: ~47kDa.
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