KLH Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Product Overview

• Article Name: KLH Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-403-466
• Supplier Catalog Number: 600-403-466
• Alternative Catalog Number: ROC-600-403-466
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Species Reactivity: Mollusc
• Immunogen: KLH (Keyhole Limpet Hemocyanin
• Conjugation: HRP
• Alternative Names: rabbit Anti-KLH antibody peroxidase conjugation, HRP conjugated rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:400,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-KLH Antibody Peroxidase Conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of KLH in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) substrate for 30 minutes at room temperature. A

Images

Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody (600-401-466) at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TBS overnight at 4C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.