This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with methylated lysine conjugated KLH.
Conjugation:
HRP
Alternative Names:
Dimethylysine antibody, Methyl lysine antibody, N epsilon dimethyl lysine antibody, N epsilon mono methyllysine antibody, N epsilon trimethyl lysine antibody, N(epsilon) trimethyl lysine antibody, Trimethyl lysine antibody
Clonality:
Polyclonal
Concentration:
1 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Liquid (sterile filtered)
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:5,000, WB: 1:1,000-1:5,000
Application Notes:
This affinity-purified antibody is suitable for use in ELISA and western blotting. The antibody reacts specifically with methylated lysine residues. Although not tested, this antibody is likely functional in RIA, flow cytometry and immunohistochemistry.
Western Blot of Anti-Lysine Methylated (MeK) Antibody Peroxidase Conjugated. Load: HeLa whole cells. Primary Antibody: Anti-Lysine Methylated HRP Antibody. Lane A: Signal methylated proteins without blocking. Lane B: Signal methylated proteins blocked using 50µg/mL methylated BSA. Lane C: Signal methylated proteins blocked using 10µg/mL methylated BSA.
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