GST Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal

Product Overview

• Article Name: GST Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-405-200
• Supplier Catalog Number: 600-405-200
• Alternative Catalog Number: ROC-600-405-200
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA
• Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.
• Conjugation: ALP
• Alternative Names: rabbit anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated rabbit anti-GST antibody, Glutathione-S-Transferase

Product Properties

• Clonality: Polyclonal
• Concentration: 0.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000-1:10,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
• Application Notes: Anti-Glutathione-S-Transferase Alkaline Phosphatase Conjugate has been tested by ELISA and is suitable for western blotting and immunohistochemistry as well as other antibody based assays requiring lot-to-lot consistency.

Images

Western Blot of Rabbit Anti-GST Antibody. Lane 1: MW markers. Lane 2: recombinant GST protein (p/n 000-001-200). Load: 0.25 µg. Primary Antibody: Anti-GST at 1:1000 at room temperature for 30 min. Secondary Antibody: HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in blocking buffer (p/n MB-070). Blocking: 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C.