Human Serum Albumin Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Human Serum Albumin Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-033
• Supplier Catalog Number: 600-406-033
• Alternative Catalog Number: ROC-600-406-033
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human
• Immunogen: Human Serum Albumin
• Conjugation: Biotin
• Alternative Names: rabbit anti-human serum albumin antibody biotin conjugation, biotin conjugated rabbit anti-HSA antibody, biotin conjugated rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody biotin conjugation, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 213
• UniProt: P02768
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:60,000, IHC: 1:500 - 1:3,000, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-HUMAN SERUM ALBUMIN (RABBIT) Antibody Biotin Conjugated antibody has been tested in ELISA, western blot, and suitable in immunohistochemical assays. Optimal concentrations should be determined by researcher.

Images

Western Blot of Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated. Lane 1: Human Serum Albumin. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human Serum Albumin Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 66 kDa, 60 kDa for Human Serum Albumin.

ELISA Results of Rabbit Anti-HSA Biotin Conjugated Antibody tested against purified human serum albumin. Each well was coated in duplicate with 1.0 µg of HSA. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03) and TMB substrate (p/n TMBE-1000).