Collagen Type III Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Collagen Type III Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-105
• Supplier Catalog Number: 600-406-105
• Alternative Catalog Number: ROC-600-406-105
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Bovine, Human
• Immunogen: Collagen Type III from human and bovine placenta
• Conjugation: Biotin
• Alternative Names: rabbit anti-Collagen Type III antibody biotin conjugation, biotin conjugated rabbit anti-Collagen Type III antibody, Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody, COL3A1, Collagen alpha-1 (III) chain

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 1281
• UniProt: P02461
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Mammalian
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: 1:200 - 1:1,000, IP: 1:100, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, and western blotting for highly se

Images

Rockland anti collagen III antibody (600-401-105 Lot 26016, 1:400, 45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval. Images provided courtesy of LifeSpan Biosciences, Seattle, WA