Fibronectin Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Fibronectin Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-117
• Supplier Catalog Number: 600-406-117
• Alternative Catalog Number: ROC-600-406-117
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IHC, WB
• Species Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.
• Conjugation: Biotin
• Alternative Names: rabbit anti-Fibronectin antibody biotin conjugation, biotin conjugated rabbit anti-Fibronectin antibody, FN1, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/ml by UV absorbance at 280 nm
• NCBI: 2335
• UniProt: P02751
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000 - 1:20,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:5,000
• Application Notes: Anti-Fibronectin (rabbit) antibody was tested by WB, ELISA, and IHC. Assay by immunoblot was found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000. Assay against 1.0 µg of Fibronectin in a standard capture ELISA using Peroxidase C

Images

Immunohistochemistry of Rabbit Anti-Fibronectin Antibody. Tissue: human kidney at pH9 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibronectin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).

Immunohistochemistry of Rabbit Anti-Fibronectin Antibody. Tissue: human kidney at pH6 at 20x and 40x. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Fibronectin antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Fibronectin is cytoplasmic. Staining: Fibronectin as precipitated brown signal (A) with purple nuclear counterstain. With corresponding negative control (B).

Immunohistochemistry with rabbit anti fibronectin biotin conjugated at 20X with negative controls (right). Tissue: kidney. Fixation: FFPE buffered formalin 10% conc. Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (top) or EDTA pH 9.5 Pressure Cooker (bottom). Primary antibody: 2ug/ml for 1 hour room T. Secondary antibody: Streptav. Conj. HRP 10 ug/ml circa 45 min. room T. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Unfolding profile of Biotin Conjugated Rabbit Anti-Fibronectin Antibody. The fluorescence signal is plotted against temperature. The vertical line indicates the Ti at 63.4C. Independently Validated byantibodies-online GmbH (p/n ABIN5596762) courtesy of NanoTemper Technologies.