Phosphothreonine Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Phosphothreonine Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-263
• Supplier Catalog Number: 600-406-263
• Alternative Catalog Number: ROC-600-406-263
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Other
• Immunogen: PhosphoThreonine affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphothreonine conjugated KLH.
• Conjugation: Biotin
• Alternative Names: PhosphoThreonine (pT) reactive antibody PhosphoThreonine (pT) polyclonal antibody, PhosphoThreonine antibody produced in rabbit

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000, IHC: User Optimized, IP: User Optimized, WB: 1:500
• Application Notes: PhosphoThreonine affinity-purified antibody is tested for use in ELISA and western blotting assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine

Images

Western Blot of Anti-PhosphoThreonine (pT) Antibody Biotin Conjugated. Load: SP2/0 myeloma cells activated with FBS 10mins after serum free starvation overnight (Lane A: 12µL and Lane B: 4µL whole cell lysates).Primary Antibody: Anti-PhosphoThreonine (pT) Antibody Biotin Conjugated. Secondary Antibody: Rabbit Anti-Biotin Peroxidase Conjugated.