6X His Tag Antibody Biotin Conjugated, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-600-406-382
Supplier Catalog Number:
600-406-382
Alternative Catalog Number:
ROC-600-406-382
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Conjugation:
Biotin
Alternative Names:
rabbit anti-6X HIS EPITOPE TAG Antibody biotin conjugation, biotin conjugated rabbit anti-6X HIS EPITOPE TAG Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Clonality:
Polyclonal
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or
Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 µg/ml was used to detect 1.0 µg of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code 611-132-122) for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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