DYKDDDDK Tag (Anti-FLAG) Antibody Biotin Conjugated, Rabbit, Polyclonal
Biozol Catalog Number:
ROC-600-406-383
Supplier Catalog Number:
600-406-383
Alternative Catalog Number:
ROC-600-406-383
Manufacturer:
Rockland Immunochemicals
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG conjugated proteins.
Conjugation:
Biotin
Alternative Names:
rabbit antibody for the detection of FLAG(TM) conjugated proteins biotin conjugated, rabbit anti DYKDDDDK Biotin
Clonality:
Polyclonal
Concentration:
1.0 mg/mL by UV absorbance at 280 nm
Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
This antibody is optimally suited for monitoring the expression of FLAG(TM) tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG(TM) epitope. The antibody recognizes the epitope tag fused to the carboxy or
Rocklands antibody to detect FLAG(TM) conjugated proteins is shown to detect as little as 3 ng of amino-terminal FLAG(TM) tagged recombinant protein by western blot. This antibody was used at a 1:1,000 dilution to detect 3-fold serial dilutions of amino-terminal FLAG(TM)-Bacterial Alkaline Phosphatase (BAP) fusion protein (Sigma P-7582) starting at 1.0 µg of protein as shown in lanes 1-6 respectively. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) (code 611-132-122) for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Affinity Purified Antibody to detect FLAG(TM) conjugated proteins detects both C terminal linked and N terminal linked FLAG(TM) tagged recombinant proteins by western blot. This antibody was used at a dilution of 1:2,500 to detect 1.0 µg of recombinant protein containing either the FLAG(TM) epitope tag linked at the carboxy (C) or the amino (N) terminus of the recombinant protein. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (code 611-132-122) for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
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