KLH Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: KLH Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-466
• Supplier Catalog Number: 600-406-466
• Alternative Catalog Number: ROC-600-406-466
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Mollusc
• Immunogen: KLH (Keyhole Limpet Hemocyanin
• Conjugation: Biotin
• Alternative Names: rabbit Anti-KLH antibody biotin conjugation, biotin conjugated rabbit Anti-KLH antibody, Keyhole Limpet Hemocyanin

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:250,000, IHC: User Optimized, WB: 1:5,000 - 1:25,000
• Application Notes: Anti-KLH Biotin conjugated Antibody has been tested by western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Anti-KLH antibody biotin conjugate is suitable for use with streptavidin reporter systems. Researcher

Images

Western Blot of Rabbit anti-KLH antibody. Lane 1: KLH reduced. Lane 2: none. Load: 1 µg per lane. Primary antibody: KLH antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TTBS for 30 min RT. Predicted/Observed size: 350-390 kDa for KLH. Other band(s): none.