GDF15 Antibody Biotin Conjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GDF15 Antibody Biotin Conjugated, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-600-406-B07
• Supplier Catalog Number: 600-406-B07
• Alternative Catalog Number: ROC-600-406-B07
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, WB
• Species Reactivity: Human, Mouse
• Immunogen: Nag-1 Antibody Biotin Conjugated antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein. A residue of cysteine was added to facilitate coupling to KLH.
• Conjugation: Biotin
• Alternative Names: rabbit anti-NAG1 antibody biotin conjugation, biotin conjugated rabbit anti-NAG1 antibody, NAG 1, NAG-1, GDF15, MIC1, MIC-1, GDF-15, PLAB, PTGFB, nonsteroidal anti-inflammatory drug-activated gene, NSAID-activated gene 1 protein, growth differentiation factor 15, macrophage inhibitory compound 1, Placental bone morphogenetic protein, Prostate differentiation factor

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 9518
• UniProt: Q99988
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Application Notes: Nag-1 Antibody Biotin Conjugated antibody has been tested by ELISA and western blotting of human and mouse NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-ter

Images

Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific). Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.

Immunohistochemistry of Rabbit anti NAG1 antibody (C-terminal specific). Tissue: Human Colon at 20X in colon tissue at pH 6. Negative control of human colon tissue pH6 is shown in background.

Western blot using Rocklands affinity purified anti-NAG-1/GDF15 (C-terminal) antibody shows detection NAG-1 purified from CHO cells as a 14 kDa band corresponding to monomer and a 28 kDa band corresponding to dimerized NAG-1. Samples were electrophoresed on a 4-20% gradient gel under reducing conditions. Lane 1 shows NAG-1 detection. Lane 2 shows reactivity is blocked when this unconjugated antibody is pre-incubated with the immunizing peptide prior to Western blotting.