0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Form:
Lyophilized
Antibody Type:
Primary Antibody
Application Dilute:
ELISA: 1:60,000, Flow Cytometry: User Optimized, IF Microscopy: User Optimized, WB: 1:2,000 - 1:10,000
Application Notes:
Anti-GST Texas Red Conjugated Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Western Blot showing detection of recombinant GST protein (0.25 µg) in lane 2. MW markers are in lane 1. Protein was run on a 4-20% gel, then transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4C primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit (p/n 611-103-122) secondary antibody was used at 1:40,000 in MB-070 blocking buffer and imaged on the VersaDoc(TM) MP 4000 imaging system (Bio-Rad).
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