Anti-Cyanine (Sheep) Antibody, Polyclonal

Product Overview

• Article Name: Anti-Cyanine (Sheep) Antibody, Polyclonal
• Biozol Catalog Number: ROC-600-601-209
• Supplier Catalog Number: 600-601-209
• Alternative Catalog Number: ROC-600-601-209
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: ELISA
• Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
• Conjugation: Unconjugated
• Alternative Names: sheep anti Cyanine Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:50,000, IHC: User Optimized, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Cyanine (Sheep) Antibody has been tested by ELISA and is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.

Images

ELISA of results purified Sheep anti-Cyanine Antibody. Each well was coated in duplicate with 1.0 µg of BSA-conjugated Cy5. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using blocking buffer MB-060-1000, Donkey anti-Sheep HRP conjugate 1:10,000, and TMB-8000 substrate.