Dopamine Beta-Hydroxylase Antibody, Sheep, Polyclonal

Product Overview

• Article Name: Dopamine Beta-Hydroxylase Antibody, Sheep, Polyclonal
• Biozol Catalog Number: ROC-600-601-D28
• Supplier Catalog Number: 600-601-D28
• Alternative Catalog Number: ROC-600-601-D28
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: WB
• Species Reactivity: Human, Primate
• Immunogen: Anti-Dopamine beta-Hydroxylase Antibody was produced in sheep by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.
• Conjugation: Unconjugated
• Alternative Names: Dopamine beta-monooxygenase, DBH

Product Properties

• Clonality: Polyclonal
• NCBI: 1621
• UniProt: P09172
• Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
• Form: Liquid
• Target: Human
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000, WB: 1:1000
• Application Notes: Anti-Dopamine beta-Hydroxylase Antibody is tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 75 kDa in size corresponding to DBH protein in the appropriate

Images

Western Blot of Anti-Dopamine ß-Hydroxylase (Sheep) Antibody. Lane 1: human adrenal medulla lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Dopamine ß-Hydroxylase antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) sheep secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~75kDa/~75kDa for DBH protein. Other band(s): none.