Cyanine Antibody Alkaline Phosphatase Conjugated, ALP, Sheep, Polyclonal

Product Overview

• Article Name: Cyanine Antibody Alkaline Phosphatase Conjugated, ALP, Sheep, Polyclonal
• Biozol Catalog Number: ROC-600-605-209
• Supplier Catalog Number: 600-605-209
• Alternative Catalog Number: ROC-600-605-209
• Manufacturer: Rockland Immunochemicals
• Host: Sheep
• Category: Antikörper
• Application: DOT, ELISA
• Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
• Conjugation: ALP
• Alternative Names: sheep anti-Cyanine Antibody alkaline phosphatase Conjugation, alk phos conjugated sheep anti-Cyanine Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Cyanine Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and dot blot. This product has been assayed against 1.0 ug of Cyanine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 mi

Images

Dot Blot of Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated. Antigen: Row 1 - Cy3 Streptavidin Conjugated Row 2 - Cy5 Streptavidin Conjugated. Load: Lane 1 - 200 ng Lane 2 - 66.67 ng Lane 3 - 22.22 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Primary antibody: none. Secondary antibody: Sheep anti-Cyanine Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 1 HR at RT. Visualized using NBT-100 Alkaline Phosphatase Substrate for 30 seconds at RT.