Rabbit IgG anti-Bovine IgG (H+L)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Bovine IgG (H+L)-unconj., MinX none
• Biozol Catalog Number: ROC-601-4102
• Supplier Catalog Number: 601-4102
• Alternative Catalog Number: ROC-601-4102
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Bovine
• Immunogen: Bovine IgG whole molecule
• Conjugation: Unconjugated
• Alternative Names: rabbit anti-Bovine IgG Antibody, Rb-a-Bovine IgG, Bovine IgG Antibody in Rabbit, rabbit anti Bovine IgG
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Bovine IgG (H&L) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot consistency.

Images

ELISA Results of Purified Rabbit Anti-Bovine IgG Antibody tested against purified Bovine IgG. Each well was coated in duplicate with 1.0 µg of Bovine IgG (p/n 001-0102). The working dilution is 1:420,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugation Stabilizer (p/n MB-076), Goat Anti-Rabbit HRP conjugated (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).