Rabbit IgG anti-Bovine IgG (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Bovine IgG (Fc)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-601-4503
• Supplier Catalog Number: 601-4503
• Alternative Catalog Number: ROC-601-4503
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Bovine
• Immunogen: Bovine IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: rabbit anti-Bovine IgG F(c) alkaline phosphatase Conjugated Antibody, rabbit anti-Bovine IgG Fc alk phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Bovine
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Bovine F(c) Alkaline Phosphatase Conjugate has been tested by ELISA. This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code NPP-10 as a substrate for 30 minutes at room temper

Images

ELISA Results of Purified Rabbit Anti-Bovine IgG F(c) Antibody Alkaline Phosphatase Conjugated tested against purified Bovine IgG F(c) Alk Phos. Each well was coated in duplicate with 1.0 µg of Bovine IgG F(c) (p/n 001-0103). The working dilution is 1:5300. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).