Rabbit IgG anti-Cat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Cat IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-602-4502
• Supplier Catalog Number: 602-4502
• Alternative Catalog Number: ROC-602-4502
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Feline
• Immunogen: Cat IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: Rabbit Anti-Cat IgG Antibody Alkaline Phosphatase Conjugation, Rabbit Anti-Cat IgG Alk Phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Cat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:10,000 - 1:20,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Cat IgG Alkaline Phosphatase conjugate has been tested by ELISA and dot blot. This product is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assa

Images

ELISA Results of Rabbit Anti-Cat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Cat IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Cat IgG (p/n 002-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).