Rabbit IgG anti-Cat IgG (F(ab)2)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Rabbit IgG anti-Cat IgG (F(ab)2)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-602-4504
• Supplier Catalog Number: 602-4504
• Alternative Catalog Number: ROC-602-4504
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Feline
• Immunogen: Cat IgG F(ab)2 fragment
• Conjugation: Alk. Phos.
• Alternative Names: Rabbit Anti-Cat IgG F(ab)2 Alkaline Phosphatase Conjugated Antibody, Rabbit Anti-Cat IgG Fab2 Fragment Antibody Alk Phos conjugation
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Cat
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Cat IgG F(ab)2 Alkaline Phosphastase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lo

Images

ELISA results of purified Rabbit Anti-Cat IgG F(ab)2 Alkaline Phosphatase Conjugated Antibody tested against Cat IgG F(ab)2. (Red Line) Each well was coated in duplicate with 1.0µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000) and TMB substrate (p/n TMBE-1000).