Chicken IgG (H&L) Antibody Rhodamine Conjugated, TRITC, Goat, Polyclonal

Product Overview

• Article Name: Chicken IgG (H&L) Antibody Rhodamine Conjugated, TRITC, Goat, Polyclonal
• Biozol Catalog Number: ROC-603-100-002
• Supplier Catalog Number: 603-100-002
• Alternative Catalog Number: ROC-603-100-002
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: DOT
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: TRITC
• Alternative Names: goat anti-Chicken IgG Rhodamine Conjugated Antibody, goat anti-Chicken IgG Antibody TRITC Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY Rhodamine Conjugated Antibody, goat anti-Chicken IgY Antibody TRITC Conjugation

Product Properties

• Clonality: Polyclonal
• Concentration: 2.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: Anti-Chicken IgG Rhodamine Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, i

Images

Western Blot of Unconjugated Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.