Chicken IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed, TRITC, Goat, Polyclonal

Product Overview

• Article Name: Chicken IgG (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed, TRITC, Goat, Polyclonal
• Biozol Catalog Number: ROC-603-100-126
• Supplier Catalog Number: 603-100-126
• Alternative Catalog Number: ROC-603-100-126
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: TRITC
• Alternative Names: goat anti-Chicken IgG Rhodamine Conjugated Antibody, goat anti-Chicken IgG Antibody TRITC Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY Rhodamine Conjugated Antibody, goat anti-Chicken IgY Antibody TRITC Conjugation

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Images

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG/IgY whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.