Goat IgG anti-Chicken IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Product Overview

• Article Name: Goat IgG anti-Chicken IgG (H+L)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Biozol Catalog Number: ROC-603-105-002
• Supplier Catalog Number: 603-105-002
• Alternative Catalog Number: ROC-603-105-002
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: Alk. Phos.
• Alternative Names: Goat anti-Chicken IgG Antibody Alkaline Phosphatase Conjugation, Goat anti-Chicken IgG Alk Phos Conjugated Antibody, Goat anti-Chicken IgY Antibody Alkaline Phosphatase Conjugation, Goat anti-Chicken IgY Alk Phos Conjugated Antibody
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Form: Liquid (sterile filtered)
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Application Notes: Anti-Chicken IgG Alkaline Phosphatase conjugated antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunoelectron microscopy and immunohistochemistry as well as other antibody based enzymatic assays requiring lot-to-lot co

Images

Western Blot of Unconjugated Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG/IgY (H&L) (GOAT) Antibody (p/n 603-101-002) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab. Chicken F(c) migrates slightly higher.