Goat IgG anti-Chicken IgM (µ)-Biotin, MinX none

Product Overview

• Article Name: Goat IgG anti-Chicken IgM (µ)-Biotin, MinX none
• Biozol Catalog Number: ROC-603-106-007
• Supplier Catalog Number: 603-106-007
• Alternative Catalog Number: ROC-603-106-007
• Manufacturer: Rockland Immunochemicals
• Host: Goat
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgM whole molecule
• Conjugation: Biotin
• Alternative Names: goat anti-Chicken IgM (mu chain) Antibody Biotin Conjugation, goat anti-Chicken IgM mu chain biotin Conjugated Antibody
• Format: IgG
• Target Specificity: IgM (µ)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Application Notes: Anti-Chicken IgM Biotin Conjugated Antibody has been tested by ELISA. This product has been assayed against 1.0 ug of Chicken IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline

Images

Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007) 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.