Chicken IgG Fc Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal

Product Overview

• Article Name: Chicken IgG Fc Antibody Rhodamine Conjugated, TRITC, Rabbit, Polyclonal
• Biozol Catalog Number: ROC-603-4003
• Supplier Catalog Number: 603-4003
• Alternative Catalog Number: ROC-603-4003
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Species Reactivity: Gallus
• Immunogen: Chicken IgG F(c) fragment
• Conjugation: TRITC
• Alternative Names: rabbit anti-Chicken IgG F(c) Antibody Rhodamine Conjugation, rabbit anti-Chicken IgG F(c) TRITC Conjugated Antibody, rabbit anti-Chicken IgY F(c) Antibody Rhodamine Conjugation, rabbit anti-Chicken IgY F(c) TRITC Conjugated Antibody

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Application Notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platfor

Images

Western Blot of Unconjugated Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.