Rabbit IgG anti-Chicken IgG (Fc)-unconj., MinX none

Product Overview

• Article Name: Rabbit IgG anti-Chicken IgG (Fc)-unconj., MinX none
• Biozol Catalog Number: ROC-603-4103
• Supplier Catalog Number: 603-4103
• Alternative Catalog Number: ROC-603-4103
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG F(c) fragment
• Conjugation: Unconjugated
• Alternative Names: rabbit anti Chicken IgG F(c) Antibody, rabbit anti-Chicken IgG Fc Antibody, rabbit anti-Chicken IgY F(c)
• Format: IgG
• Target Specificity: IgG (Fc)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 2.1 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Liquid (sterile filtered)
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:20,000 - 1:100,000, IHC: 1:1000-1:5000, WB: 1:2000-1:10,000
• Application Notes: Anti-Chicken IgG F(c) antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Images

Western Blot of Unconjugated Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(c) (RABBIT) Antibody (p/n 603-4103) 1:2,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.