Rabbit IgG anti-Chicken IgG (F(ab)2)-HRPO, MinX none

Product Overview

• Article Name: Rabbit IgG anti-Chicken IgG (F(ab)2)-HRPO, MinX none
• Biozol Catalog Number: ROC-603-4304
• Supplier Catalog Number: 603-4304
• Alternative Catalog Number: ROC-603-4304
• Manufacturer: Rockland Immunochemicals
• Host: Rabbit
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG F(ab)2 fragment
• Conjugation: HRPO
• Alternative Names: rabbit anti-Chicken IgG F(ab)2 Antibody Peroxidase Conjugation, rabbit anti-Chicken IgG Fab2 Fragment HRP Conjugated Antibody, rabbit anti-Chicken IgY F(ab)2 HRP
• Format: IgG
• Target Specificity: IgG (F(ab')2)
• Cross-Adsorption (MinX): no cross-adsorbtion

Product Properties

• Clonality: Polyclonal
• Concentration: 1.5 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:40,000 - 1:90,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Chicken IgG F(ab)2 Peroxidase conjugate has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection sh

Images

Western Blot of Unconjugated Anti-Chicken IgG F(ab)2 (RABBIT) Antibody (p/n 603-4104). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG F(ab)2 (RABBIT) Antibody (p/n 603-4104) 1:5,000 for 60 min at RT. Secondary antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and F(ab). Chicken F(c) migrates slightly higher.