Donkey IgG anti-Chicken IgG (H+L)-HRPO, MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal

Product Overview

• Article Name: Donkey IgG anti-Chicken IgG (H+L)-HRPO, MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, Polyclonal
• Biozol Catalog Number: ROC-603-703-C37
• Supplier Catalog Number: 603-703-C37
• Alternative Catalog Number: ROC-603-703-C37
• Manufacturer: Rockland Immunochemicals
• Host: Donkey
• Category: Antikörper
• Application: ELISA,IHC,WB
• Species Reactivity: Gallus
• Immunogen: Chicken IgG whole molecule
• Conjugation: HRPO
• Alternative Names: Donkey anti-Chicken IgG Antibody Peroxidase Conjugation, Donkey anti-Chicken IgG HRP Conjugated Antibody, Donkey anti-Chicken IgY Antibody Peroxidase Conjugation, Donkey anti-Chicken IgY HRP
• Format: IgG
• Target Specificity: IgG (H+L)
• Cross-Adsorption (MinX): Bovine,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat,Sheep

Product Properties

• Clonality: Polyclonal
• Concentration: 1.0 mg/mL by UV absorbance at 280 nm
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Form: Lyophilized
• Target: Chicken
• Antibody Type: Secondary Antibody

Application Notes

• Application Dilute: ELISA: 1:65,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Application Notes: Anti-Chicken IgG (H&L) Peroxidase has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be opt

Images

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (DONKEY) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-701-C37) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Donkey IgG (GOAT) Peroxidase Conjugated Antibody (p/n 616-1302) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.